Functional promotion of phytoremediation by Cucurbitaceae-plant growth promoting bacteria complex system

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K12379
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 64020:Environmental load reduction and remediation-related
• Research Institution: University of Yamanashi
• Principal Investigator: Kataoka Ryota 山梨大学, 大学院総合研究部, 准教授 (00635104)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: Cucumber / endophytic bacteria / Plant growth promoting / phytoremediation

Outline of Final Research Achievements

The diversity of endophytic bacteria in cucumber was investigated by both cultured and non-cultured methods. Certain bacteria were always present inside the cucumber, and in some cases, it has seemed to be selected by the cucumber. In addition, about 300 endophytic bacterial strains were isolated from the starks of cucumber, and the possibility of PGP was investigated. PGP selected 5 strains of the isolates that showed the better results for plant growth promotion and further investigated using pot experiments. Strains 4 and 227 showed particularly better results and were also found to increase fruit number and growth under field experiments. Although the two strains showed common PGP characteristics, metabolomics analysis suggested that the mechanism for the plant growth promoting effect was fundamentally different.

Free Research Field

土壌学および土壌微生物学

Academic Significance and Societal Importance of the Research Achievements

DDTとPCP（ペンタクロロフェノール）で汚染された土壌を植物と微生物を用いて浄化するための新しい浄化技術が提案された。つまり、土壌中に低濃度かつ広範囲に広がる汚染を効率よく効果的に除去するために植物を用いた除去（ファイトレメディエーション）に加えて植物の生育を促進する内生細菌を組み合わせることで、ファイトレメディエーションの強化を図るものである。ファイトレメディエーションを微生物で機能強化した新たな汚染物質除去技術は、ファイトレメディエーションに寄与する内生細菌の多様性解析から分離・選抜、機能解析を通じて植物‐微生物間相互関係や微生物の生存戦略などに関する興味深い知見を与えるものである。