Development of real-time confocal phase-contrast microscope and observation of tissue cells

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K12769
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: Osaka Electro-Communication University
• Principal Investigator: Hisaka Masaki 大阪電気通信大学, 医療健康科学部, 教授 (40340640)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 位相差顕微鏡

Outline of Final Research Achievements

Phase-contrast microscopy is regarded as an advanced and important basic research field. In recent years, it has become especially important as a method to observe cells, such as iPS cells, while they are still alive. In this study, we have developed a new confocal phase contrast microscope. This microscope enables simultaneous observation of absorption and transparent refractive index images of tissue cells, and also reduces the effects of lens-induced image distortion and multiple scattering of light within the cells.

Free Research Field

医用光学

Academic Significance and Societal Importance of the Research Achievements

共焦点位相差顕微鏡は，フーリエ空間における空間周波数抽出フィルタを適用することで，観察試料の位相構造のみを選択的に観察することができます．さらに，画像再生に必要な回折光のみを効率的に抽出することで，組織細胞内で生じる多重散乱光の多くを除去できる特徴を持ちます．この顕微鏡は，iPS細胞観察だけでなく，表層組織および表層近傍に発生した癌細胞などの早期発見にも適用可能な新しい医療診断機器として期待できます．