Cell type-independent surface modification for cell-cell adhesion

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K12805
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90120:Biomaterials-related
• Research Institution: Kyushu University
• Principal Investigator: Arima Yusuke 九州大学, 先導物質化学研究所, 准教授 (90402792)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 細胞表面修飾 / 細胞間接着 / 核酸 / タンパク質

Outline of Final Research Achievements

Cell-cell adhesion plays important roles in biomedical applications such as the construction of native-like tissues. In this study, we aimed to establish cell type-independent control of cell-cell adhesion.
Amphiphilic molecules carrying peptide nucleic acid with neutral backbone allowed for the modification of surfaces of many cell types, and thus the induction of adhesion to another cell types through hybridization.
We also tested cell-cell adhesion through cell surface modification with proteins. Modification of cell surface with ligand enabled to bind to proteins through binding of ligand to specific sequence in protein and thus to adhere to another cell types.

Free Research Field

バイオマテリアル

Academic Significance and Societal Importance of the Research Achievements

生体類似組織の形成や抗体作製などの医学応用において，細胞の接着が不十分なため使用可能な細胞種の限定や効率の低さなどが問題であった。本技術を用いることで多種多様な細胞種に対して表面修飾が可能となり，細胞を扱う医学・生物学分野における細胞間接着の問題を解決する方法を提供できる。
また，細胞種による表面修飾効率の違いを生じさせる原因を知ることで，種々の分子が混在する細胞表面に関する更なる地検が得られると期待できる。