Molecular level evaluation of cell culture substrate and construction of nano-controlled surface toward cell culture standardization

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K12814
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90120:Biomaterials-related
• Research Institution: National Institute for Materials Science (2020-2021); Foundation for Advancement of International Science (2019)
• Principal Investigator: SHIBATA-SEKI Teiko 国立研究開発法人物質・材料研究機構, 先端材料解析研究拠点, NIMS特別研究員 (90773309)
• Co-Investigator(Kenkyū-buntansha): 赤池 敏宏 公益財団法人国際科学振興財団, その他部局等, 主席研究員 (30101207) ; 後藤 光昭 公益財団法人国際科学振興財団, その他部局等, 主任研究員 (80235001)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 細胞培養 / 細胞培養基質 / カドヘリン / AFM / SPM / QCM

Outline of Final Research Achievements

The surface of the dish was coated with the cell-recognizing chimeric antibody E-cadherin-Fc (E-cad-Fc) protein, and the adsorption state and functional expression of the surface were elucidated, and its possibility as a substrate coat standardization material was clarified. The adsorption state of the dish surface of E-cad-Fc was analyzed by submerged AFM, and it was proposed that it could be easily used as an index of the coating state by using not only the image but also the adsorption force between the AFM probe and the sample. We also succeeded in visualizing the state of homophyllic bonds between cadherins on a nanoscale. Furthermore, by combining AFM and QCM　measurement , it was possible to investigate the orientation of molecules in the liquid. It was confirmed that the material and surface condition of the cell culture substrate is important, and the research results of E-cad-Fc here will be useful for future cell culture.

Free Research Field

SPM　表面

Academic Significance and Societal Importance of the Research Achievements

再生医療の基礎となる細胞培養基質の標準化は、バイオマテリアルの発展に繋がり、人類の福祉に貢献する。