Development of an ultra-sensitive simultaneous enzyme immunoassay for porcine proteins compatible with the Halal system

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K14034
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 08030:Family and consumer sciences, and culture and living-related
• Research Institution: Kochi Gakuen University (2021); University of Kochi (2019-2020)
• Principal Investigator: NUMATA Satoshi 高知学園大学, 健康科学部, 講師 (10565857)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 酵素免疫測定法 / ハラル制度 / 豚肉

Outline of Final Research Achievements

Pork is considered a contraindicated food in Islam. This study aimed to develop an ultra-sensitive enzyme immunoassay to detect pork with high sensitivity. Porcine serum albumin and porcine myoglobin were selected as antigens. The development of an ultra-sensitive enzyme immunoassay requires two types of antibodies for an antigen. But, only one antibody each for porcine serum albumin and porcine myoglobin could be obtained. Therefore, the ultra-sensitive enzyme immunoassays for porcine serum albumin and porcine myoglobin are unfinished. If we can obtain the remaining antibodies in the future, we believe it will be possible to develop ultra-sensitive enzyme immunoassays for porcine serum albumin and porcine myoglobin.

Free Research Field

給食経営管理

Academic Significance and Societal Importance of the Research Achievements

本研究は、イスラム教徒にとって禁忌食品である豚肉を誤って摂取しないようにするため、加工食品中の豚由来成分を高感度に検出する新たな手法として、超高感度酵素免疫測定法の開発を試みた。しかし、完全な超高感度酵素免疫測定法の開発までには至らなかった。
今後の課題として残りの抗体を作成し、超高感度酵素免疫測定法の開発を実現するよう試みる。