Visualization of facilitated diffusion of transcription-factor by utilizing novel high-speed super-resolution microscopy

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K15406
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 28040:Nanobioscience-related
• Research Institution: Center for Novel Science Initatives, National Institutes of Natural Sciences
• Principal Investigator: Tsutsumi Motosuke 大学共同利用機関法人自然科学研究機構(新分野創成センター、アストロバイオロジーセンター、生命創成探究, 生命創成探究センター, 特任助教 (60782422)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 深部超解像イメージング / 高速超解像イメージング / 生体イメージング / 先端蛍光顕微鏡

Outline of Final Research Achievements

Aiming to visualize intracellular molecular behavior in living cells occurring in nano-scale area at several ten milli-seconds, I established a super-resolution observation method that enables high-speed and deep observation. By utilizing SRRF, a novel super-resolution method based on image analysis, and applying it to our research group's advanced two-photon excitation fluorescence microscope, I demonstrated super-resolution observation with several ten milli-seconds temporal resolution at millimeter-order depths in living organisms and tissues. In addition, in this study, I established a remote super-resolution observation system with a collaborator. These results were presented at several conferences and international meetings, and awarded a conference presentation award. In addition, three co-authored papers were published in academic journals as the results of joint research utilizing the findings obtained in this study.

Free Research Field

生物物理

Academic Significance and Societal Importance of the Research Achievements

本研究で確立された高速・深部超解像観察法は、転写因子の高速DNA認識動態の観察のみならず、脳をはじめとする生体組織、臓器、３次元培養細胞など、既存超解像顕微鏡法では観察困難な対象における微細分子挙動や形態観察に適用でき、生命科学研究の発展に貢献する。また、本研究に関連して得られた細胞スフェロイド培養法やその２光子観察法の知見は薬剤活性の評価モデルとして有用であり、国際共同研究を含む複数の研究に応用されている。さらに、本研究で実証したリモート顕微鏡観察の系は、感染症蔓延下の対応だけではなく、遠隔地に住む研究者が先端顕微鏡観察の機会を得られる点から、海外顕微鏡共用施設の運営者からも注目されている。