Cell observation platform using biological nanopore probes

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K15418
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 28050:Nano/micro-systems-related
• Research Institution: Nagaoka University of Technology
• Principal Investigator: Shoji Kan 長岡技術科学大学, 技学研究院, 准教授 (80788258)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: ナノポア / 走査型イオンコンダクタンス顕微鏡 / 構造DNAナノテクノロジー / 脂質二分子膜 / 生体ナノポアプローブ

Outline of Final Research Achievements

In this project, in order to realize the measurement of chemicals released from living cells, we developed biological nanopore probes that can detect local chemicals and demonstrated to detect biological chemicals with the biological nanopore probes by controlling the position of the probe with scanning ion conductance microscopy. As a result, we successfully developed the nanopore probe with nanometer scale tip size and mapped biological molecules using the probe. In addition, we developed a DNA nanopore probe that synthetic DNA nanopores are modified on a gold needle electrode and successfully offered the potential of spatially-resolved chemical detection using the DNA nanopores. These results were published in several journals including ACS Nano in the ACS.

Free Research Field

ナノマイクロシステム

Academic Significance and Societal Importance of the Research Achievements

本研究の学術的意義は、プローブ型の人工細胞膜システムを開発することで、これまで均一なバルク溶液中にのみ応用されてきたナノポアセンシングによる分子検出を局所的な分子検出に応用し、ナノポアセンシングによる局所分子検出の特性について検証した点である。一方、社会的意義は、本研究成果により細胞分泌物質のリアルタイム計測が可能となることで、細胞分泌が寄与する様々な疾患のメカニズム解明や創薬に寄与できることである。今後、本技術を発展させ細胞分泌物質計測の実現を目指す。