2021 Fiscal Year Final Research Report
Generation of super-bright fluorescent RNA and visualization of intracellular RNA dynamics
| Project/Area Number |
19K15720
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 37030:Chemical biology-related
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Furuhata Yuichi 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (40828026)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 核酸 / 蛍光RNA / RNA分析 / 蛍光相関分光法 |
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| Outline of Final Research Achievements |
To obtain bright fluorescent RNAs, we had planned to express a partially randomized library of the fluorescent RNA sequence in human cells and screen them with fluorescence. In the detailed analysis of the fluorescent RNA we developed, which is the basis of the randomized library, we obtained valuable insights into the relationship between the structure of the fluorescent RNA and its fluorescence mechanism. Thus, we altered our research objective to establish a new analytical method to evaluate the mechanism of high fluorescence and the properties of RNAs that contribute to high fluorescence, and established this method.
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| Free Research Field |
バイオテクノロジー
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では，蛍光RNAが持つ配列・構造とその蛍光特性との関係性について詳細に理解するため，蛍光相関分光法に基づいた方法論を開発するとともに，既報の蛍光RNAの蛍光特性を再評価した．本法の開発により最先端のRNAイメージングツールである蛍光RNAの特性を一分子レベルで解析し，その発光効率や分子論的性質に関する踏み込んだ評価が可能となった．本解析プラットフォームは多様化と改良が加速している蛍光RNAツールのベンチマークに有効であり，RNAに起因する幅広い疾患や生命現象の解明に貢献することが期待される．
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