Analysis of submergence stress response in Nucleus-Cytoplasmic interaction of common wheat

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K15820
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: Ryukoku University
• Principal Investigator: Takenaka Shotaro 龍谷大学, 農学部, 講師 (20757736)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: コムギ / オルガネラ / 冠水耐性

Outline of Final Research Achievements

The cultivation of common wheat in paddy shifting fields, difficulty with drainage, requires submergence and/or waterlogging tolerant varieties that guarantee uniform and good seedling growth. In this study, we conducted a comprehensive search for set of genes differentially expressed in seedlings of nucleus-cytoplasm substitution lines (having wheat nuclei and cytoplasm of a closely related species, Aegilops, hereafter abbreviated as NC lines), which have been reported to show different responses to submergence stress. We could detect 531 differentially expressed genes between variety Chinese Spring and the NC line. These genes set were not observed in the control plots. The results suggested that they are related to the nucleus-cytoplasm interaction with submergence stress.

Free Research Field

植物遺伝育種

Academic Significance and Societal Importance of the Research Achievements

コムギ・エギロプス属から作出された核細胞質置換雑種系統は，核ゲノムと細胞質ゲノムの間の相互作用を解析するための貴重な実験材料である。しかし，これまでに農業に有効利用されている知見は，細胞質雄性不稔と核の稔性回復遺伝子を利用した一代雑種のみであった。先行研究によって，細胞質が冠水ストレス応答性にかかわっていることが明らかになっていたが，その具体的な機構は未解明である。本研究では，核細胞質置換雑種系統において，冠水ストレス応答に具体的にどのような遺伝子群がかかわっているのかを明らかすることができた。