Hisotological analysis of mouse embryo implantation with tissue clearing method

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K15965
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: Kitasato University
• Principal Investigator: Sugiyama Makoto 北里大学, 獣医学部, 助教 (30648225)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 胚着床 / マウス / 妊娠 / 発生学

Outline of Final Research Achievements

The implantation site in mice is not predictable. As this result, “when” and “how” the murine embryos reach implantation in the uterus remains undefined. This question needs to be clarified to provide a complete picture of the implantation phenomenon. From this background, we applied the tissue clearing method to pregnant mice to trace mouse embryos. We then identified a detailed time schedule for the embryo implantation period in the murine pregnancy. Furthermore, in the process of our research, we established and reported two novel methods, tissue clearing, and a highly successful of producing pregnant mice.

Free Research Field

獣医解剖学

Academic Significance and Societal Importance of the Research Achievements

本研究により、マウス胚着床現象の解明に向けた基礎的な情報基盤を得ることができた。その結果、本研究分野で最も重要な課題である「胚着床期に由来する習慣性流産」の克服を期待する、実験動物モデルを構築することができたと考える。また、本研究を遂行するに当たり確立した組織透明化方は、汎用性の高く他分野への応用が可能である。さらに、同時に確立した高効率の交配適期マウスの新規選定法もまた、生殖科学・実験動物学分野への広い貢献を期待できる。