Pathological and genetic study of inclusion body disease of Japanese brown cattle.

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K15994
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: WATANABE Kenichi 帯広畜産大学, 畜産学部, 助教 (10761702)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 褐毛和種牛 / SOD1 / 神経変性疾患 / 封入体

Outline of Final Research Achievements

We examined 8 cases of Inclusion body disease of Japanese Brown cattle (IBD) and 208 slaughtered healthy brains (collected from healthy Japanese brown cattle reared in same area). Intraneuronal inclusions were found in all of IBD cases and approximately 30% of the slaughtered brain. The inclusions were immuno-positive for SOD1, but SOD1 gene mutations were not found in these individuals. Although there was no histological difference between slaughtered brains with/without inclusions, RNA-seq revealed a significant upregulation of mitochondria-related molecules in cases with inclusions. These findings suggest that these inclusions are "true" pathological findings due to mitochondrial abnormalities or abnormal aggregation of SOD1.

Free Research Field

獣医病理学

Academic Significance and Societal Importance of the Research Achievements

IBDの病理像はSOD1の凝集などヒトのALSとの共通点が多く、発現解析の結果などは関連疾患の研究にも還元可能な知見であると言える。また、封入体形成がみとめられた健常牛については発症以前の段階あるいは無症状の保因牛であるとも考えられることから、神経変性疾患の病態発生を考える上で貴重なサンプルと言える。IBDの認知度は低く、発生地域もごく限られているが、過去には死亡例なども発生しており、貴重な和牛の系統である褐毛和種牛の血統維持を考える上でIBDのさらなる病態解明が望まれる。