Development of BioID mouse models for an innovative in vivo interactome analysis

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16019
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 42040:Laboratory animal science-related
• Research Institution: University of Tsukuba
• Principal Investigator: Murata Kazuya 筑波大学, 医学医療系, 助教 (60713485)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: タンパク質間相互作用 / in vivo BioID

Outline of Final Research Achievements

Protein-protein interaction (PPI) has a critical role for the proper regulation of cellular and tissue functions. In this study, we have generated a novel BioID mouse model, which enables us to directly analyze PPI in vivo. We investigated an appropriate method for biotin administration and found that feeding a 0.5% biotin diet efficiently induced biotinylation of proximal proteins. This method can avoid unnecessary chemical solvent-induced biological changes and injection-related stresses in mice.

Free Research Field

生化学

Academic Significance and Societal Importance of the Research Achievements

in vivo BioIDは基礎生物学、分子生物学、医学など多岐にわたる分野で活用が見込まれる強力な研究ツールであり、本研究成果はin vivo BioIDの基盤となる新規ビオチン化誘導法を報告したものである。また、in vivo BioIDは各種組織における利用例が少ない状況であったが、脳や心臓、肝臓、精巣など幅広い組織で活用可能であることを示した。