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2021 Fiscal Year Final Research Report

Elucidating the mechanism underlying the mesenchymal polarization of the trachea through Wnt signaling

Research Project

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Project/Area Number 19K16156
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 44020:Developmental biology-related
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Kishimoto Keishi  国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (70700029)

Project Period (FY) 2019-04-01 – 2022-03-31
Keywords気管 / 極性化 / 間充織 / Wnt
Outline of Final Research Achievements

In this study, we attempted to explore the Wnt-related genes which are expected to be involved in mesenchymal polarization of the trachea. scRNA-seq transcriptomics and its validation with in situ hybidization have identified several Wnt signaling components during mesenchymal polarization. We also tried to establish the system in which the process of mesenchymal polarization can be analyzed in vitro. We have succeeded in generating tracheal mesenchyme from human/mouse embryonic stem cells. We are currently combining these mesenchyme with epithelial cells to recapitulate the process of tracheal mesenchymal polarization.

Free Research Field

発生生物学

Academic Significance and Societal Importance of the Research Achievements

我々は先行研究において、気管が正しい長さと太さに成長するためには、間充織細胞の極性化が必要であることを見出した。一方、これまでの当該分野の研究の多くは上皮細胞に焦点をあっており、間充織組織の発生についてはあまり研究されていなかった。本研究では、呼吸器間充織細胞をヒト幹細胞から作成することを世界に先駆けて成功した。これらの細胞を上皮細胞と混合培養することで、ヒト臓器の形成過程を培養皿上で再構築する技術の確立に繋がる。これらの技術はヒト臓器の正常な形成機構の解明・病態の理解につながると期待される。

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Published: 2023-01-30  

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