Development of genetically-encoded voltage indicator for plants

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K16164
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 44030:Plant molecular biology and physiology-related
• Research Institution: Nagoya University
• Principal Investigator: Yoshinari Akira 名古屋大学, 高等研究院, 特任助教 (00829872)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: シロイヌナズナ / 膜電位 / GEVI / イメージング

Outline of Final Research Achievements

This study aimed to establish a membrane potential imaging system using existing membrane potential sensors and newly developed sensors, so-called genetically-encoded voltage indicators (GEVIs), in order to obtain a more detailed understanding of the important membrane potentials in plant physiology. This study demonstrated the necessity of using GEVIs in plant cell membrane potential imaging that are less affected by pH and provided insights for future Plant-GEVI development.

Free Research Field

植物生理学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果により、将来のPlant-GEVI開発の指針が示された。また、研究の過程で得られた数多くの新規GEVIは、動物細胞においては良好な応答を示しており、神経細胞における膜電位イメージングなどへの応用が期待される。