Generation of a zebrafish model for elucidating effects on the early development and regeneration of prostaglandin

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16354
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 47030:Pharmaceutical hygiene and biochemistry-related
• Research Institution: Tokyo University of Science, Yamaguchi
• Principal Investigator: Tsuge Kyoshiro 山陽小野田市立山口東京理科大学, 薬学部, 助教 (40825121)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: ゼブラフィッシュ / 脂質 / プロスタグランジン / 再生医療 / GPCR

Outline of Final Research Achievements

In this study, we performed comprehensive analyses of prostaglandin (PG) receptor-deficient zebrafish lines and revealed that the PGE2-EP3 receptor pathway promotes lymphatic vessel differentiation through the induction of lymphatic vessel differentiation factor expression in venous endothelial cells. And then, we have also found important receptor pathways for kidney development and gonad development, and are currently researching detailed mechanisms. Furthermore, we have discovered that lipid mediators related to PG may be involved in organ regeneration, and are currently in the process of identifying responsible receptors and analyzing biochemical properties.

Free Research Field

生化学、分子生物学、発生生物学、薬学、再生医療学

Academic Significance and Societal Importance of the Research Achievements

本研究は、これまでほとんど明らかにされていなかったPGの初期発生作用の分子機構を解明したものであり、学術的意義が大きい。本研究は、NSAIDsの適正使用や脂質メディエーターを用いた再生医療の基盤となるものであり、社会的意義も大きいと考えられる。