Molecular mechanism analysis of fatty acid-binding protein in intracellular pharmacokinetics

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16458
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 47060:Clinical pharmacy-related
• Research Institution: Suzuka University of Medical Science
• Principal Investigator: Yamamoto Atsushi 鈴鹿医療科学大学, 薬学部, 助教 (90633991)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 脂肪酸結合タンパク質 / FABP / 変異体解析 / 薬物結合 / 薬物代謝

Outline of Final Research Achievements

While the drug-binding proteins in blood plasma such as albumin have been investigated in detail, the proteins in the cells have been hardly studied. It is thought that fatty acid-binding protein (FABP) binds various drugs and affects the pharmacokinetics in the cells. In this study, we synthesized the recombinant proteins of FABP mutant and performed drug-binding analysis. To elucidate the influence of FABP on drug metabolism, we performed knockdown experiments using siRNAs against FABP1, and constructed the separation analysis of the drug metabolism.

Free Research Field

生物薬剤学

Academic Significance and Societal Importance of the Research Achievements

多くの薬物は生体内では基本的にタンパク質と結合して運搬されており、この結合は薬効発現や副作用惹起と深く関わる。本研究では、脂肪酸結合タンパク質（FABP）が細胞内における薬物動態に与える影響を解明し、細胞内薬物動態学という新たな学術分野を切り開くとともに、薬物の作用発現や副作用惹起のメカニズムをより深く理解することを目的としている。FABPの変異体解析や薬物代謝に与える実験系の構築を行い、FABPの薬物結合研究における基盤を築いた。