Elucidation of the physiological significance of platelet-derived microparticles

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16545
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49010:Pathological biochemistry-related
• Research Institution: Josai University (2020-2021); Hoshi University (2019)
• Principal Investigator: Yusuke Ando 城西大学, 薬学部, 助教 (10805881)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: マクロファージ / 血小板 / 細胞外小胞 / エクソソーム / NASH / 血液 / miRNA / EVs

Outline of Final Research Achievements

In this study, we aimed to elucidate the physiological significance of qualitative and quantitative fluctuations of extracellular vesicles (EVs) in circulating blood, which are expected as biomarkers for various pathological conditions, and focused on the changes in the phenotype of macrophages. The results suggested that blood EVs induce phenotypic changes in macrophages via blood, and changes in miRNA cargo of EVs, which caused by the formation of various pathological conditions, regulate macrophage infection protection. In addition, it was considered that the changes in blood EVs were affected by the qualitative and quantitative fluctuations of platelets, which are the sources of EVs.

Free Research Field

免疫学

Academic Significance and Societal Importance of the Research Achievements

本研究では、生体恒常性の維持に重要な役割を担うマクロファージの形質変化を通して、EVsの評価ならびにEVs含有miRNA中の病態マーカーの探索を行なった。本研究で得られた知見は、NASHのみならずマクロファージの異常に起因する多くの病態形成の理解に繋がると考えられる。