2020 Fiscal Year Final Research Report
A molecular mechanism of breast cancer cell-derived exosomes using protein-protein interactions
| Project/Area Number |
19K16548
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 49020:Human pathology-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | 乳癌 / エクソソーム / 血管新生 |
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| Outline of Final Research Achievements |
In this study, we immunolocalized the exosome markers CD63, CD81, CD9 and Caveolin-1 in breast cancer tissues and then correlated the findings with microvessel density (MVD) evaluated by CD31 immunohistochemistry. In addition, the levels of CD9-positive cancer cells were also significantly higher in those with high proliferation marker protein Ki-67. We then demonstrated exosomes derived from Cav-1 knockdown-MDA-MB-231 cells increased HUEhT-1 tube formation. Our results indicated that exosome markers and Caveolin-1 positive cancer cells could be involved in clinically and biologically aggressive subtypes of breast cancer, suggesting that it may be involved in angiogenesis and cell proliferation.
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| Free Research Field |
人体病理学，腫瘍学
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| Academic Significance and Societal Importance of the Research Achievements |
癌細胞由来エクソソームは血清などを用いてその意義が広く検討されているが，本研究では，通常病理診断で用いられるホルマリン固定パラフィン包埋組織においてエクソソームマーカーの発現を検討し，癌細胞での発現が高い症例では血管新生が促進しており，より悪性度が高いことを示した．本研究により，エクソソームの新たな解析手法を提案し，また病理検査技術や治療法の開発にも寄与することが期待される．
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