Generation of gene-knockout library in Chlamydia pneumoniae

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K16660
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49050:Bacteriology-related
• Research Institution: Fukuoka University
• Principal Investigator: Shimizu Akinori 福岡大学, 医学部, 講師 (40780135)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: 肺炎クラミジア / グループIIイントロン / 遺伝子改変 / プラスミドベクター

Outline of Final Research Achievements

I tried to establishing a gene manipulated-Chlamydia pneumoniae library previously unreported. I carried out to construct the plasmid DNA vector which I designed at first. The DNA sequences were confirmed and same as designed sequences, however the transformant clones of C. pneumoniae were not obtained. To solve this problem, previous DNA samples were check by RFLP patterns. Then, the RFLP patterns were different from re-purified plasmid DNA that were same subclones before more than a month. The reason why the RFLP patterns were changed was not uncoverd. This result shown that difficulty of establishing a gene manipulated-C. pneumoniae library by the method that I tried.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

今回の研究結果として、グループIIイントロンを利用した遺伝子改変プラスミドベクターの構築は問題なく達成できた。しかしながら、少なくとも今回設計したグループIIイントロンのプラスミドベクターは短期間しか保存することができなかった。