2021 Fiscal Year Final Research Report
Elucidation of clinical significance and metastasis mechanism of circulating tumor cells undergoing EMT in lung cancer
Project/Area Number |
19K16786
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 50010:Tumor biology-related
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Research Institution | University of Occupational and Environmental Health, Japan |
Principal Investigator |
NABE YUSUKE 産業医科大学, 医学部, 非常勤医師 (10813264)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | 循環腫瘍細胞 / 上皮間葉移行 / 肺癌 |
Outline of Final Research Achievements |
The microfluidic chip "CTC-chip" (CSV-chip), which is combined with Cell Surface Vimentin (CSV) specifically expressed on tumor cells undergoing epithelial-mesenchymal transition (EMT), has well capture efficiency in tumor cells with strong mesenchymal characteristics. This system was considered to be capable of capturing EMT-CTCs. In a study of surgical cases, CTCs were captured by the conventional EpCAM-chip and CSV-chip before surgery in three cases of recurrence, and in two of these cases, CTCs were captured only by the CSV-chip in the CTC examination immediately before recurrence, indicating that the CSV-chip can capture CTCs with high metastatic potential. It is expected that the CSV-chip can be used to collect CTCs with high metastatic potential.
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Free Research Field |
呼吸器外科
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Academic Significance and Societal Importance of the Research Achievements |
原発巣から放出された腫瘍細胞が血流に乗って他臓器へ到達することにより転移が生じると考えられている。その血液中の腫瘍細胞のことを循環腫瘍細胞(CTC)といい、CTCが存在する症例では今後転移を生じる危険性が高いと考えられる。従来までCTCを検出するターゲットとして、上皮細胞接着分子(EpCAM)が用いられてきたが、血液中にがんが入るためには上皮間葉移行(EMT)を生じ、上皮系性質のEpCAMの発現が下がり、間葉系性質のマーカーの発現が増加すると考えられている。本研究では転移形成に重要な役割を担うEMTを生じたCTCを高感度に捕捉するシステムの構築を図り、その有用性を示唆することができた。
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