Discovery of an inhibitor for exosome secretion in cancer cells using a small-molecule library approach.

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K16848
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 50020:Tumor diagnostics and therapeutics-related
• Research Institution: Tokyo Medical University
• Principal Investigator: Yoshioka Yusuke (吉岡祐亮) 東京医科大学, 医学部, 講師(特任) (60721503)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: エクソソーム / 細胞外小胞 / がん / がんの転移 / 低分子化合物

Outline of Final Research Achievements

This study focused on the screening of small-molecule inhibitors for exosome secretion in ovarian cancer cells. Screening system is based on ExoScreen assay for monitoring CD9 positive EV secretion. Using this screening system and a chemical compound library inhibitors for exosome secretion were identified in the ovarian cancer cell line ES-2. As a result of the screening, some small molecules were selected as candidate molecules for inhibitors of exosome-secretion and some molecules inhibited exosome secretion in a cancer cell specific manner.

Free Research Field

分子腫瘍学

Academic Significance and Societal Importance of the Research Achievements

本研究で得られた成果は、卵巣がん細胞が分泌するエクソソームを介した細胞間コミュニケーションを断つ方法の一つとして、新たながん転移予防法へと繋がる可能性がある。また選択された化合物はすでにFADにおいて承認されている化合物であるため、実用化への道のりを目指しやすい。本方法を用いることで卵巣がんのみならず、様々ながん種および疾患へと応用可能である。