Usefulness of AST and ALT measurement using pyridoxal phosphate in patients with vitamin B6 deficiency.

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K16961
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 52010:General internal medicine-related
• Research Institution: Juntendo University
• Principal Investigator: Idei Mayumi 順天堂大学, 医学部, 准教授 (40596623)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: トランスアミナーゼ / ビタミンB6 / ピリドキサールリン酸

Outline of Final Research Achievements

AST and ALT activities are widely measured using the JSCC methods without addition of pyridoxal phosphate (PALP) in Japan. This study investigated the usefulness of AST and ALT measurement using the IFCC method with addition of PALP in patients with vitamin B6 deficiency. The measurement of AST using the IFCC method was unstable, therefore, ALT was investigated. Patients with low vitamin B6 levels had a higher rate of low ALT activities, and the ALT increase rate ((IFCC-JSCC)/JSCC) was significantly higher in the low ALT group than in control group. This suggests the usefulness of ALT measurement using the IFCC method with addition of PALP in patients with low ALT activities. In this study, the increase in ALT activities measured by the IFCC method did not significantly affect the assessment of liver damage.

Free Research Field

臨床検査

Academic Significance and Societal Importance of the Research Achievements

AST、ALTの測定は、国際臨床化学連合の勧告法（IFCC法）ではPALPを添加し測定されるが、国際的にはPALPを添加することは統一されていない。本邦では、PALP非添加のJSCC標準化準拠法（JSCC法）で測定することが一般的であり、ビタミンB6欠乏状態では、AST、ALTが見かけ上低値となり、肝障害を過小評価する可能性がある。本研究により、ビタミンB6低値ではALT低値の割合が高く、ALT低値例において、IFCC法測定の有用性が示された。