2020 Fiscal Year Final Research Report
Examination of biological meaning of change in oxygen status and oxygen gradation in chronic kidney disease
Project/Area Number |
19K17697
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 53040:Nephrology-related
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Research Institution | The University of Tokyo |
Principal Investigator |
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Project Period (FY) |
2019-04-01 – 2021-03-31
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Keywords | 低酸素 / 低酸素応答因子 / 尿細管細胞 |
Outline of Final Research Achievements |
In this research, the importance of oxygen gradient in cultured renal tubular cells were examined. The applicant established culture method of tubular cells which contained oxygen gradient, and examined distribution of hypoxia-inducible factor (HIF). It is widely known that in homogenous oxygen tension, HIF accumulates inversely correlated to oxygen tension. However, the applicant found that HIF decreased in extreme hypoxia near anoxia, and also found that low pH attenuated HIF accumulation in extreme hypoxia. Combined together, it was proposed that both oxygen status and acid-base balance are closely involved in the accumulation of HIF. These results were published as a paper(Physiol Rep. 2021 ;9:e14689).
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Free Research Field |
腎臓内科学
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Academic Significance and Societal Importance of the Research Achievements |
慢性腎臓病は未だ根治が得られていない疾患である。慢性腎臓病の進行には腎臓における低酸素が密接に関与することが既に知られているが、生体内において、実際にどの部位が低酸素を生じており、そして生体内で低酸素がどのような影響をもたらすのかは未だ明らかになっていない。申請者は、生体内酸素イメージング法を用いて生体の腎臓において酸素勾配が存在することを明らかにしており、これまでの培養細胞を用いた結果が正しく生体内の事象を模しているかに疑問を持った。本研究は、より生体を模した細胞の培養法を用いた検討で、従来の培養細胞とは異なる結果が得られており、今後の培養細胞を用いた検討のあり方に一石を投じるものである。
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