2021 Fiscal Year Final Research Report
Development and evaluation of rapid molecular diagnostic test for drug-restistant bacteria in urine samples
Project/Area Number |
19K17923
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 54030:Infectious disease medicine-related
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Research Institution | Kyoto University |
Principal Investigator |
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | 薬剤耐性菌 / 迅速診断 |
Outline of Final Research Achievements |
Appropriate empiric antimicrobial treatment for urinary tract infections is challenging in the era of ESBL-producing Enterobacterales that exhibit multidrug-resistance including key treatment drugs of third-generation cephalosporins. However, appropriate rapid diagnostic tests are not available for clinical use. In this study, we developed a direct molecular testing method for rapid detection of ESBL-producing Enterobacterales from urine. A total of 272 clinical urine specimens were used for validation of the test. The test had a sensitivity of 96% and a specificity of 100%, indicating validity and reliability of the developed test.
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Free Research Field |
臨床検査医学
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Academic Significance and Societal Importance of the Research Achievements |
本検査法の臨床での使用により、迅速・高精度・簡便・安価に検査室や臨床現場において、1時間以内に薬剤耐性菌感染症の有無の判定が可能となる。これにより、尿路感染症の治療において、薬剤耐性菌に対する早期の適切な治療と感受性菌であった場合双方において適切な抗菌薬選択が可能となり、患者の疾患治療成績の向上および、抗菌薬適正使用を通じた薬剤耐性菌の抑制に貢献できる見込みである。
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