2019 Fiscal Year Annual Research Report
Generation of HIV-1 resistant T cells from induced pluripotent stem cell using CRISPR/Cas9 system
| Project/Area Number |
19K17925
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| Research Institution | Kobe University |
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Principal Investigator |
オフィンニ ユディル 神戸大学, 医学研究科, 教育研究補佐員 (30837901)
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| Project Period (FY) |
2019-04-01 – 2020-03-31
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| Keywords | HIV-1 / iPSC / CRISPR/Cas9 / T cell / Feeder-free / Lentivirus |
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| Outline of Annual Research Achievements |
Step 1, "establish anti-HIV-1 CRISPR/Cas9", has went very well. We have developed gRNAs targeting HIV-1 regulatory genes, which inhibit
replication in infected T cells. Multiplexed attack on HIV-1 tat blocks viral escape for 40 days, keeping the cells immune in long-term. Step 2,
"transduce iPSC with CRISPR/Cas9", is going well. We used lentiviral transductions to insert genes into iPSC before antibiotic selection, which
was successful. However, off-target screening needs to be done to determine the safety. Step 3, "to differentiate iPSC into T cells immune to
HIV-1", is ongoing. We are developing a feeder-free culture system to differentiate iPSC into HSC and T cells. But, cell differentiation takes a
very long time, whether CRISPR is functional remains to be seen, and delay is expected.
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Research Products
(4 results)
