2022 Fiscal Year Annual Research Report

Generation of HIV-1 resistant T cells from induced pluripotent stem cell using CRISPR/Cas9 system

Research Project

Project/Area Number	19K17925
Research Institution	Osaka University
Principal Investigator	オフィンニユディル大阪大学, 免疫学フロンティア研究センター, 特任研究員(常勤) (30837901)
Project Period (FY)	2021-03-01 – 2023-03-31
Keywords	HIV-1 / iPSC / CRISPR/Cas9 / T cell
Outline of Annual Research Achievements	Step 1 of the study has been published in Viruses in 2020 and selected as a cover story of the journal's #11 issue. In Step 2, I have successfully transduced Cas9-gRNA into human mobilized hematopoietic stem cells (HSC) using a lentivector with Cocal virus pseudotyped-envelope. But, Step 3 did not go well as I moved to the US (HIV lab in Harvard), Osaka (Immunology), and now Kobe (Clinical Virology). The labs were not an iPSC lab. iPSC differentiation needed strict protocol and culture environment, and I did not succeed in differentiating the transduced iPSC. I am still hoping to establish a position in an iPSC lab to continue the project. I prematurely ended the KAKENHI project as I was adopted as a Program-Specific Assistant Professor in Kobe and cannot have any extra research effort.

(3 results)