2021 Fiscal Year Final Research Report
Functional analysis of novel kininogen-derived peptides identified by plasma peptideome technology
| Project/Area Number |
19K18013
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 54040:Metabolism and endocrinology-related
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | ブラジキニン / 新規ペプチド |
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| Outline of Final Research Achievements |
We have succeeded in developing a "human plasma peptideome technology" that discover low molecular weight peptides present in human plasma using mass spectrometry. When peptides with sequences not recorded in all existing peptide databases were chemically synthesized and added to purchased cultured cells to search for cellular responses such as increased intracellular calcium concentration and gene proliferation, two types of sequences derived from kininogen were found. A strong intracellular signal-inducing effect was observed in the peptides of the above, and an antagonistic effect on bradykinin was also observed in two different peptides.
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| Free Research Field |
内分泌学
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| Academic Significance and Societal Importance of the Research Achievements |
ブラジキニン、あるいは生体内に存在するその類似物質の解明は、人体における疼痛や掻痒、時には生命に関わる急性アレルギー疾患などといった治療に難渋する疾患・病態の診断や治療に有益である。
当大学が開発・改良した技術によって、「生理活性をもった新規キニノーゲン由来ペプチドがあり、それらが確実にヒト血中に存在している」ことがわかった。本研究の遂行が未解明の疾患・病態に一石を投じ、創薬を含めた新たな治療法開発につながるなどの波及効果が期待できる。
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