Comprehensive genetic analysis of fatty liver gafts for marginal donor expansion

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K18140
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Ishii Takeshi 東京医科歯科大学, 東京医科歯科大学病院, 医員 (10837058)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 虚血再灌流 / 肝動脈 / 脂肪肝

Outline of Final Research Achievements

We studied the changes in gene expression in hepatocytes following inhibition of the hepatic artery in rats. Although there have been many studies on the simultaneous inhibition of portal vein and hepatic artery blood flow, there have been no studies focusing on the inhibition of hepatic artery blood flow alone. In this study, we performed a comprehensive gene analysis using RNA microarray. The results showed that among the genes expressed by arterial clamping, those in the GTP metabolic pathway varied widely, suggesting that they are likely to be involved in ischemia-reperfusion injury. Since the GTP metabolic pathway is thought to contribute to the generation of nitric oxide synthase NOS and thus to ischemia-reperfusion injury, our experimental system is valid and further analysis is warranted.

Free Research Field

肝胆膵外科

Academic Significance and Societal Importance of the Research Achievements

肝動脈の阻血に対する肝類洞組織の応答、つまりは遺伝子発現の変化について詳細かつ客観的な網羅的解析を行うことで虚血再灌流に対する肝類洞組織の生理学的応答の詳細なメカニズムの解明につながる。このことは最終的には肝移植治療の卑近の問題であるドナー不足とマージナルドナーの拡大の問題の解決につながると考えられる。