Post-VAD implant cardiac remodeling: molecular biological analysis focusing on Fhod3, cytoskeleton-related protein

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K18187
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55030:Cardiovascular surgery-related
• Research Institution: Kyushu University
• Principal Investigator: Ushijima Tomoki 九州大学, 大学病院, 助教 (70529875)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 心不全 / 心筋症

Outline of Final Research Achievements

Sarcomere-associated proteins (Fhod3, cMYBP-C, β-MHC, actin), cardiac hypertrophy-related factors (ANP, BNP, Ark, Akt), nuclear transfer-associated pathway (SRF/MRTF), Ca handling-related factors (Serca2, Phospholamban), mitochondrial metabolism-related factors (TFAM) and fibrosis-related factors (Periostin, Collagen) were evaluated quantitatively and qualitatively by immunostaining, mRNA analysis with real-time PCR , immunoblotting. There was no significant and notable difference between the individuals. No correlation between the expression level of these factors and the clinical data was found.

Free Research Field

心不全

Academic Significance and Societal Importance of the Research Achievements

本研究では、分子生物学的視点から、補助人工心臓治療の治療効果を判定するための有用な知見は得られなかった。より多くの症例数での解析で、新たな結果を導くことがきるかもしれない。今後も心筋細胞レベルで心不全治療の効果を判定する試みは重要であると考える。