Elucidation of the link between the environmental change in the intestinal flora and cognitive decline due to anesthetic surgery

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K18247
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55050:Anesthesiology-related
• Research Institution: Sapporo Medical University
• Principal Investigator: Tachibana Shunsuke 札幌医科大学, 医学部, 助教 (30737309)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 腸内フローラ / 術後認知機能障害 / dysbiosis

Outline of Final Research Achievements

The primary objective of this study was to elucidate the mechanisms and effects on cognitive function mediated by intestinal flora during the perioperative period.
Using a group of mice that were assumed to be in the perioperative period, we analyzed the variation in intestinal flora diversity. The RNA was converted to cDNA and then analyzed individually by qRT-PCR with reference to candidate genes selected by transcriptome analysis in our previous study.
Surgical and/or anesthetic invasions on older mice may affect changes in RNA expression in the brain.

Free Research Field

神経麻酔

Academic Significance and Societal Importance of the Research Achievements

多くの高齢者が手術麻酔を受ける現代において、周術期の認知機能低下、行動異常などを発症する原因を同定する可能性がある。また、これらの脳内での微細な変化は、事前にこれら現象発症を予測するためのマーカーともなりうるだろう。