Pathophysiology of burn-induced blood vitamin D depletion and efficacy of vitamin D supplementation

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K18364
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Keio University
• Principal Investigator: Sato Yukio 慶應義塾大学, 医学部(信濃町), 講師 (00445272)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 熱傷 / ビタミンD / グルタミン / アラニン

Outline of Final Research Achievements

The relationship between vitamin D and glutamine, glucose intolerance, and immunocompromise in burns is unclear. In this study, using a mouse model of burns with 20% burn area, we performed metabolomic analysis of metabolite changes induced by high-dose vitamin D administration. The results showed that metabolites in the amino acid metabolic pathway and TCA circuit in the gastrocnemius muscle, metabolites involved in ATP synthesis and degradation, and glutamine and alanine levels in the blood were increased in the burn group treated with high-dose vitamin D administration. Blood levels of glutamine, which is necessary for immunomodulation and wound healing, and blood levels of alanine, which is necessary for gluconeogenesis in the liver, were increased, suggesting that glutamine and alanine may contribute to the improvement of immunosuppression and impaired glucose tolerance.

Free Research Field

救急医学

Academic Significance and Societal Importance of the Research Achievements

熱傷は骨量減少、耐糖能異常、筋委縮、免疫低下を来たす。本研究は臨床研究報告がされている熱傷患者の予後と血中ビタミンD濃度に相関性が認められることについての病態解明を目指した。本研究では熱傷モデルマウスへのビタミンD投与は、免疫調節および創傷治癒に必要なグルタミンの血中濃度および肝臓における糖新生に必要なアラニンの血中濃度が増加し、免疫調節や耐糖能異常の改善に寄与する可能性が示唆され、臨床研究で報告されている熱傷へのビタミンDの影響の一因と考えられた。