2022 Fiscal Year Final Research Report
New treatment method for brain hemorrhage by co-culturing neural stem/progenitor cells using olfactory ensheathing cells
Project/Area Number |
19K18387
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 56010:Neurosurgery-related
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Research Institution | Osaka University |
Principal Investigator |
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Project Period (FY) |
2019-04-01 – 2023-03-31
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Keywords | iPS細胞 / 神経幹/前駆細胞 / シート化 / 脳出血モデル |
Outline of Final Research Achievements |
We planned an experiment to explore a method to regenerate brain hemorrhage by co-culturing neural stem/progenitor cells using olfactory ensheathing cells as a scaffold and shaping them into sheets. Although we were able to establish a method to create neural stem/progenitor cells via cerebral organoids, we were unable to conduct the experiment using olfactory ensheathing cells due to various reasons, so we aimed to create sheets using biomaterials instead, but were unable to succeed. We were also able to create a subcortical hemorrhage model using SD rats, but were unable to establish an immunosuppression method for transplantation.
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Free Research Field |
再生医療
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Academic Significance and Societal Importance of the Research Achievements |
神経幹細胞を用いた脳出血の再生方法に関してはほとんど研究が進んでいない。障壁としては出血部位の微小環境の悪化や広範な出血による欠損部の補填が細胞数的に困難であることが挙げられる。我々は足場を用いた神経幹細胞の培養方法の確立や、ラット脳出血モデルへの移植方法の確立を模索したもののどちらも思ったような成果が上がらず、中途終了する方針となった。しかし、本研究での神経幹細胞の樹立方法やラット脳出血モデルの確立などは今後の研究の礎になるものと期待される。
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