2021 Fiscal Year Final Research Report
Development of vagus nerve stimulation therapy for Parkinson's disease
| Project/Area Number |
19K18430
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 56010:Neurosurgery-related
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2019-04-01 – 2022-03-31
|
| Keywords | パーキンソン病 / 迷走神経刺激療法 / 神経保護効果 / 抗炎症効果 |
|---|
| Outline of Final Research Achievements |
A cuff-type silver electrode was placed in the left cervical vagus nerve and then 6-hydroxydopamine was stereotactically administered to the left striatum to create a model of Parkinson disease. VNS was performed for 14 days. The stimulation conditions are divided into 4 groups: stimulation width: 500usec, stimulation frequency: 30Hz, stimulation intensity: 0.10mA, 0.25mA, 0.50mA, 1.0mA. Result: VNS showed behavioral improvement from the 7th day with low-intensity stimulation of 0.25mA and 0.50mA, and the effect was maintained even on the 14th day. Under the same conditions, TH-positive fiber density and cell number were significantly maintained compared with the control group, and the expression numbers of inflammatory microglia and astrocytes in the same area decreased in the stimulation group. In addition, NA neurons in the locus coeruleus were retained in the stimulus group.
|
| Free Research Field |
神経科学
|
| Academic Significance and Societal Importance of the Research Achievements |
迷走神経は全身に広く分布されており、VNSの正確な機序については不明点が多いのが実情である。今後は迷走神経の上行性・下行性線維をそれぞれ刺激し、炎症性サイトカイン、アセチルコリン受容体の発現動態の評価を追加で行う予定である。近年では、PDの発症と腸管炎症の関連性、腸脳相関が注目されており、腸管炎症モデルを使用した実験も病態解明に有効と考える。また、当科はPDモデルへ新規システムを使用した脊髄刺激実験も施行している。外科的な低侵襲治療の開発に向けて、複数の電気刺激実験を並行して施行する計画である。
|
