Feeder-supported in vitro exercise model using human satellite cells

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K18484
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Tohoku University
• Principal Investigator: Koide Masashi 東北大学, 医学系研究科, 大学院非常勤講師 (70804706)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 筋衛星細胞 / サルコペニア

Outline of Final Research Achievements

Collecting atrophic and adipose-degenerated human muscle tissue and normal muscle tissue during arthroscopic shoulder surgery and isolating muscle satellite cells from them. A small fingertip-sized muscle sample was taken from the un-ruptured subscapularis muscle (normal group), and ruptured supraspinatus muscle (torn group). Muscle satellite cells (CD11b-CD31-CD34-CD45-CD56 + cells) were isolated using a FACS (Fluorescence activated cell sorting) device following collagenase treatment of small fingertip-sized muscle samples. The separated muscle satellite cells were cultured and proliferated, and cryopreserved. We constructed a contractile muscle culture system that differentiates into myotube by applying electrical stimulation.

Free Research Field

整形外科

Academic Significance and Societal Importance of the Research Achievements

高齢化率が世界最速の本邦において，筋力・筋量を保持し，自活的な生活習慣を維持することが健康寿命を引き伸ばすために重要である．筋力維持のためには効果的な運動が必要であるが，一度廃用萎縮した筋組織が正常に再生するかどうかは今なお不明である．本研究の意義は廃用萎縮した筋組織の効果的な再生方法を検討することである．