Biological significance of SOD activity as a joint marker in early and end stage osteoarthritis

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K18543
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Juntendo University
• Principal Investigator: Koike Masato 順天堂大学, 医学部, 非常勤助教 (70767574)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: 変形性膝関節症 / スーパーオキシド / 酸化ストレス / SOD活性 / 滑膜炎 / 関節液

Outline of Final Research Achievements

We aimed to evaluate the SOD activity in serum, joint fluid, cartilage, and synovial membrane samples collected from 52 patients with end-stage knee OA who underwent total knee arthroplasty. The relationship between the total SOD activity in each tissue was evaluated using Spearman’s rank correlation coefficient. The joint fluid total SOD activity was used as the objective variable, and its association with the serum, cartilage, and synovial total SOD activities was evaluated using multiple linear regression analysis. Univariate analysis revealed that joint fluid total SOD activity was positively correlated with synovial total SOD activity. Multiple linear regression analysis using joint fluid total SOD activity as the objective variable showed a positive association with synovial total SOD activity. In patients with end-stage knee OA, the state of the synovial total SOD activity is better reflected by the total SOD activity in the joint fluid than that in the cartilage.

Free Research Field

整形外科学（変形性関節症、酸化ストレス）

Academic Significance and Societal Importance of the Research Achievements

末期膝OA患者の関節液中の総SOD活性は滑膜中の総SOD活性と正の相関関係にあり、関節液中の総SOD活性は軟骨中の総SOD活性よりも滑膜の状態をより良く反映している。滑膜を標的とすることはOA治療に大きな期待が持て、将来的にはOAの治療予防の標的が軟骨ではなく滑膜に移行する可能性があることを示唆する。臨床的には外来で関節穿刺により採取した関節液総SOD活性を評価することで OA を制御したいと考えている。本研究の結果は総SOD 活性は軟骨よりも滑膜の方が高く、治療または予防のために滑膜を標的とすることでOAの進行を抑制できる可能性があることを示唆している。