2021 Fiscal Year Final Research Report
Biomarker screening of urothelial carcinoma using liquid biopsy
| Project/Area Number |
19K18575
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56030:Urology-related
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 尿路上皮癌 / 糖鎖解析 |
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| Outline of Final Research Achievements |
Locally advanced or metastatic urothelial carcinoma has a poor prognosis. Early detection is important, but accurate diagnosis requires invasive tests such as cystoscopy and ureteroscopy, and minimally invasive and effective biomarkers have not been developed. We analyzed serum glycan profiling of urothelial carcinoma patients using a comprehensive glycan profiling system. The results suggested changes in immunoglobulin-associated glycans. The glycan structures of immunoglobulins were examined, suggesting that characteristic glycans may be altered, and machine learning analysis suggested high diagnostic accuracy. Prognosis prediction by combining glycan analysis and cell-free DNA is a subject for future study.
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| Free Research Field |
泌尿器癌
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| Academic Significance and Societal Importance of the Research Achievements |
局所進行性もしくは転移性の尿路上皮癌は予後不良の疾患である。早期発見が重要であるが、正確な診断には膀胱鏡・尿管鏡などの侵襲的な検査が必要であり、低侵襲かつ有効なバイオマーカーは開発されていない。本研究は、有効なバイオマーカーのない尿路上皮癌に焦点を当て、免疫グロブリンの糖鎖変化が診断マーカーになりうる可能性を示した。免疫グロブリンの糖鎖構造を検討したところ、特徴的な糖鎖が変化している可能性が示唆され、機械学習を用いた解析から、高い診断精度が示唆された。機器の普及など課題はあるが、本研究成果の学術的意義や社会的意義は非常に大きいと思われる。
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