2023 Fiscal Year Final Research Report
Biomarkers of preterm deliveries in the first trimester: exosomal placenta-specific microRNA
| Project/Area Number |
19K18650
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56040:Obstetrics and gynecology-related
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2024-03-31
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| Keywords | 絨毛膜栄養膜 / 妊娠高血圧腎症 / 妊娠糖尿病 / バイオマーカー / エクソソーム / サイトカインアレイ / インフラマソーム |
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| Outline of Final Research Achievements |
1. Exosomes were extracted from pregnant women's plasma using ultracentrifugation. CD63 had been the most common exosome marker of pregnant women; however, it did’t express in pregnant women's plasma. Alternatively, CD9 constantly expressed and can be a novel plasma-derived exosome marker. We also showed that exosomes in women with preeclampsia significantly increased.
2. Cytokine arrays were performed using plasmas in patients with gestational diabetes mellitus and their controls. Of those, we focused on CXCL5. we examined plasma CXCL5 levels using ELISA (380 cases). No significant differences between the two groups were noted.
3. β-hydroxybutyrate (BHB) inhibits the activation of the NLRP3 inflammasome in women wih preeclampsia using trophoblast cells. It can reduce the progression of preterm delivery or preeclampsia.
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| Free Research Field |
周産期学
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| Academic Significance and Societal Importance of the Research Achievements |
妊娠初期血漿を集積し、その後に発症した妊娠合併症とコントロールを用い、バイオマーカーの探索を行った。血漿中の蛋白だけでなく、エクソソームにも研究範囲を広げ、探索した。今回明らかにした知見の中では妊婦血漿のエクソソームマーカーとして、CD9を用いることが、最も有用であることを示したことは特筆すべき事項である。従来は血漿（血清）中のエクソマーカーとしてはCD9以外を用いているものが多く、これらを再検証する必要性が示唆された。超早産例等のバイオマーカーは見付けるところまで至らず、今後の研究課題である。
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