2023 Fiscal Year Final Research Report
Elucidating the Reality of Oncogenic Human Papillomavirus Infections in Japan Using Novel PCR
| Project/Area Number |
19K18712
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56040:Obstetrics and gynecology-related
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2024-03-31
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| Keywords | HPV / 子宮頚癌 / PCR / インテグレーション |
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| Outline of Final Research Achievements |
For the survey on the actual conditions of HPV-negative cancers and HPV multiple infected cancers, analysis was conducted on 16 cases of HPV-negative cancer and 9 cases of multiple HPV infected cancer using macrodissection and a newly developed PCR method. As a result, the causative HPV for all cases of multiple infections was identified. Additionally, approximately half of the HPV-negative cancers were found to be HPV-positive. In cases showing discrepancies in the results, partial or complete loss of E1 and E2 genes was observed, suggesting a high frequency of HPV-DNA integration in these cases. Integrated HPV is known to have fewer copies of DNA compared to episomal types, and it was considered that the detection sensitivity of the HPV detection assay may be related to the cause of discrepancies.
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| Free Research Field |
婦人科腫瘍
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| Academic Significance and Societal Importance of the Research Achievements |
この研究は、HPV陰性とされたがんがHPV陽性であることを明らかにし、多重感染癌の中でも単一のHPVが癌化に寄与していることを示した。HPV偽陰性だった症例の多くは、HPVゲノムのインテグレーションが高率にみられたことは新たな知見であり、遺伝子の欠失とDNAのインテグレーションががんの進展と関係していることが示唆された。HPV単独検診や併用検診についての議論が日本でも出ているが、今後のスクリーニング法を議論するうえで、より検査感度の高いアッセイの開発が必要であることが示唆された。
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