2022 Fiscal Year Final Research Report
Analysis of Signaling Mechanisms in Acquired Middle Ear Cholesteatoa via a Bioinformatics Approach
| Project/Area Number |
19K18790
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56050:Otorhinolaryngology-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | 後天性中耳真珠腫 / Notchシグナル |
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| Outline of Final Research Achievements |
We performed polymerase chain reaction in the cholesteatoma and external auditory canal (EAC) skin samples. This was followed by immunohistochemical staining of Notch1, enhancer of split-1 (HES1) cholesteatoma and EAC skin samples, respectively. The fold change of Notch1 gene expression was lowest in cholesteatoma, with a statistically significant difference. Moreover, the fold change of HES1 expression decreased. The positive rates of Notch1 and HES1 protein expressions in the cholesteatoma were significantly lower than in the EAC skin. The decreases in Notch1 and HES1 protein expression might play an important role in the hyperproliferative character of the keratinizing squamous epithelium in cholesteatoma.
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| Free Research Field |
耳科学
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| Academic Significance and Societal Importance of the Research Achievements |
後天性中耳真珠腫における角化扁平上皮の異常増殖性に関して，Notch1とHES1の発現低下が病態生理に関与している可能性が本研究にて明らかになった．このことから，Notchシグナル関連分子に着目することで，後天性中耳真珠腫に対する新たな治療戦略がみつかる可能性がある．後天性中耳真珠腫の発症原因は未だ明らかではなく，依然として根本的治療は外科的治療のみであるが，新たな標的分子，シグナル伝達機構を解明することができれば，今後の後天性中耳真珠腫治療におけるbreak throughとなり，真珠腫に対する非外科的治療法開発へ大きな一歩となることが期待される．
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