Elucidation of the molecular biological mechanism of mechanical stress on the retina

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K18844
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56060:Ophthalmology-related
• Research Institution: Okayama University
• Principal Investigator: Shiode Yusuke 岡山大学, 医歯薬学域, 助教 (20711097)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 網膜 / ミュラー細胞 / 細胞伸展刺激 / メカニカルストレス

Outline of Final Research Achievements

Cell extension stimulation was performed using retinal pigment epithelial cells (iPS-RPE) and Muller cells (MIO-M1).
In iPS-RPE, the expression of AQP-1, which has an important function on nerve cells, was decreased by a specific extension stimulus.
In MIO-M1, cell extension and co-stimulation with cytokines (TGF-β, TNF-α) showed cell pile up, suggesting a relationship with a disease that forms a fibrous proliferative membrane in the retina. In addition, upregulation of c-Fos, one of the transcription factors, was observed by stimulation of extension under specific conditions. These results are considered to contribute to the elucidation of the transcription factors that are activated and signal transduction generated in Muller cells by the stimulation of extension.

Free Research Field

網膜

Academic Significance and Societal Importance of the Research Achievements

黄斑上膜や増殖硝子体網膜症など、網膜の疾患には網膜が牽引される疾患が多く、メカニカルストレスが病態に関与している。網膜色素上皮細胞、ミュラー細胞への伸展刺激が、細胞形態にどのような変化、発現因子の変化を生じるかを解明していくことは、網膜硝子体疾患の病態解明と新たな治療研究の発展に応用できるものと考えられる。今後は動物実験モデルにおける検討など、引き続き研究を継続することが必要である。