2021 Fiscal Year Final Research Report
Elucidation of the mechanism to control keratinization in normal stratified squamous cell and squamous cell carcinoma derived from oral mucosa
| Project/Area Number |
19K18973
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57020:Oral pathobiological science-related
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| Research Institution | Matsumoto Dental University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 角化 / 分化 / 周辺帯 / トランスグルタミナーゼ |
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| Outline of Final Research Achievements |
First, I identified loricrin as a gene that contributes to oral keratinization by performing RNA sequence analysis using normal keratinized epithelium and non-keratinized stratified squamous epithelium of the oral cavity. Next, by applying the three-dimensional culture method of the epidermis to the primary gingival epithelial cells, I created a keratinized multi-layered squamous epithelial model in which the epithelial cells are laminated and show differentiation over time. Furthermore, in this model, I thought that loricrin and its cross-linking enzyme, transglutaminase 3, were localized in the cells during the formation of the keratinized layer and contributed to keratinization. Finally, I used this three-dimensional culture to create a model of intraepithelial cancer showing a tendency to differentiate. Using these culture models, we established a method to compare normal and neoplastic differentiation / keratinization.
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| Free Research Field |
口腔病理学
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| Academic Significance and Societal Importance of the Research Achievements |
口腔の重層扁平上皮において、正常な分化・角化モデルと腫瘍性の分化・角化モデルを作成することができた。したがって、このモデルと網羅的な遺伝子解析を併用することにより、正常分化から腫瘍性分化への変化の観点から、口腔癌における腫瘍発生のメカニズムを遺伝学的に解明できる。
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