2020 Fiscal Year Final Research Report
Elucidation of the mechanism of rheumatoid arthritis exacerbation mediated by HuR and exosomes induced in periodontal tissue
| Project/Area Number |
19K18999
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57030:Conservative dentistry-related
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | 歯周病 / 関節リウマチ / ＨｕＲ / エクソソーム |
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| Outline of Final Research Achievements |
In this study, we investigated whether the production of HuR and exosomes in periodontal tissues fluctuates due to stimulation by periodontopathic bacteria.
1)HuR is a protein involved in the stabilization of mRNA. HuR in gingival epithelial cells was elevated by stimulation with periodontopathic bacteria. In addition, the production of IL-6, an inflammatory cytokine, was also increased. Similar results were observed in the periodontal tissue of periodontitis mice.
2) Exosomes are vesicles secreted from cells with a diameter of 40 nm to 100 nm and are found in body fluids such as saliva and blood. The amount of exosomes released by gingival epithelial cells was increased by stimulation with periodontopathic bacteria. An increase of inflammatory cytokines was observed by addition of exosomes to the culture of gingival epithelial cells.
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| Free Research Field |
歯周病態学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究結果より、歯周組織構成細胞が歯周病原細菌の刺激を受けることでHuRおよびエクソソームが上昇する結果を得た。また、実験マウスでも同様の傾向を示した。
歯周病は、関節の腫脹や疼痛を主症状とする関節リウマチ(RA)への関与が示唆されている。HuRの関与により上昇するIL-6やエクソソーム中に含まれる因子はRA増悪に関与する。歯周病細菌の刺激を受けている歯周病患者では、HuRやエクソソームが恒常的に上昇し、RA増悪に関与している可能性が考えられる。
このような機序が明らかになることで、歯周病治療がRA治療の一助となること、およびHuRやエクソソームを標的とした新たな治療につながることが期待される。
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