2023 Fiscal Year Final Research Report
The role of PTHrP positive cells for osseointegration
| Project/Area Number |
19K19131
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57050:Prosthodontics-related
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2024-03-31
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| Keywords | 副甲状腺関連蛋白 / オッセオインデグレーション |
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| Outline of Final Research Achievements |
For in vivo lineage-tracing experiments, we have created a new mouseline, PTHrP-creERt BAC transgenic, /and combined this line with Rosa 26-tdTomato reporter line. In this system, when tamoxifen is injected at postnatal day 3, stop sequence will be removed by the enzyme cre recombinase. As a result, the PTHrP+ cells and their descendants will express red tdTomato fluorescence protein. Therefore, we are able to specifically induce follicle cells to become red /at specific time point /and trace how these cells develop overtime. I cultured PTHrP-creER+ DF cells at P8 and subsequently isolated PTHrP-creER+ colonies and subcloned them individually. Then, We tested their self-renew ability and multipotency/ by subjecting cells to trilineage differentiation conditions. They had robust potential of self-renew ability and multi-potency. Moreover, all of them differentiated into adipocytes, osteoblasts and chondrocytes. So, PTHrP-creER+ cells have self-renew ability and multi-potency.
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| Free Research Field |
生体歯科材料
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| Academic Significance and Societal Importance of the Research Achievements |
PTHrPを発現する歯小のう細胞は主に歯根膜細胞、骨芽細胞に分化するが、成長後も長期にわたり生体内に存在し、歯周組織の維持に関与している。本研究ではPTHrPにより標識される歯小のう細胞由来の細胞が、一部間葉系細胞としての性質を保持することが明らかとなった。これらの細胞がオッセオインテグレーションの獲得や歯周組織の維持、再生に重要な役割を果たしている可能性が示唆され、今後当該研究分野においてオッセオインテグレーションの獲得や歯周組織の再生メカニズムの解明に寄与することが期待される。
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