Immobilization of novel BMP-2 peptide and RANKL-binding peptide and its regulatory effects on the bone-related cell differentiation

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K19147
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57050:Prosthodontics-related
• Research Institution: Osaka Dental University
• Principal Investigator: Shuto Takahiro 大阪歯科大学, 医療保健学部, 助教 (40804604)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: BMP-2ペプチド / RANKL結合ペプチド / インプラント / 骨芽細胞 / 破骨細胞

Outline of Final Research Achievements

In the present study, we investigated the effects of novel synthetic BMP-2 peptide and RANKL-binding peptide (W9 peptide) on osteoblast and osteoclast differentiation with the aim of developing a surface modification method that greatly contributes to the improvement of long-term stability of implant treatment. The results of this study suggest that the novel synthetic BMP-2 peptide and W9 peptide shows synergetic effects of promoting osteoblastic differentiation, and the BMP-2 peptide has no inhibiting functions of suppressing osteoclast differentiation by W9 peptide. In the case of peptide immobilization to titanium surface, the similar results were obtained. Therefore, these peptides could locally alter the balance of bone formation and resorption, so as to be favorable for titanium implants.

Free Research Field

歯学

Academic Significance and Societal Importance of the Research Achievements

In vitroではあるが本研究結果より、新規合成BMP-2ペプチドとW9ペプチドの組み合わせは、より安価で安全性が高く、オッセオインテグレーション獲得や長期の予後に好影響をもたらす可能性も大きいことが予想され、口腔インプラント治療にとって非常に有意義と考えられる。 また、本固定化法の確立ができれば、チタンだけでなく、近年インプラント材料として脚光を浴びているポリエーテルエーテルケトン(PEEK)材やジルコニアなどにも応用でき、さらには整形外科領域におけるインプラント臨床応用にも展開できると期待される。