Basic study of the bone regeneration therapy using induced pluripotent stem cells derived from human peripheral blood cells

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K19176
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Tokyo Dental College
• Principal Investigator: Kato Hiroshi 東京歯科大学, 歯学部, 助教 (10755036)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: iPS細胞 / 骨芽細胞 / 骨再生 / 末梢血

Outline of Final Research Achievements

This study aims to develop a minimally invasive bone regeneration therapy utilizing iPS cells established from human peripheral blood cells. In this research, we succeeded in establishing iPS cells from peripheral blood cells of healthy individuals and inducing osteoblasts. Animal experiments were performed using the rat calvaria bone defect model. We used the atelocollagen as scaffold. While there was almost no osteogenesis in the scaffold-only group, osteogenesis was confirmed in the cell/scaffold transplant group. Histological evaluation also showed that the new bone was considered to be living bone, suggesting that the transplanted cells had osteoinductive ability. No adverse events such as infection or tumor formation were observed.

Free Research Field

口腔外科

Academic Significance and Societal Importance of the Research Achievements

これまでに末梢血血液細胞由来iPS細胞から骨芽細胞誘導を行った報告はみられないことから、本研究では、末梢血血液細胞から樹立したiPS細胞を骨芽細胞誘導し、骨形成能を有することをin vitroおよびin vivoにて評価した。末梢血採取という比較的侵襲が小さく・簡便な手技にて細胞を取得し、それをiPS細胞とする方法は、幹細胞取得手技としては低侵襲な方法である。本研究方法を応用することで、外科処置が困難な小児患者においても適用が可能な低侵襲な骨再生療法の樹立が可能となる。