Investigation of the mechanism of Medication-related ONJ (MRONJ) using cell stress visualization mouse

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K19180
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Matsumoto Dental University
• Principal Investigator: Sadaoka Sunao 松本歯科大学, 歯学部, 講師 (80549395)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: MRONJ / クロモグラニンA

Outline of Final Research Achievements

This study aims to explore the mechanism by which oxidative stress is involved in the development of MRONJ in mice treated with osteoporosis drugs over time, we focused on the Nrf2 protein, which is used for visualization in vivo, as well as chromogranin A (ChgA), which is also used as an indicator of oxidative stress, and used it as an indicator of MRONJ in the study. The purpose of this study was to elucidate the expression of oxidative stress in mice and whether it is enhanced by bacterial factors or other factors. Since we have previously shown that ChgA gene was enhanced by osteoporosis drugs on osteoclasts, we decided to explore the effect of suppression of the Nrf2-keap1 system, which involves the Nrf2 protein, on ChgA gene expression.

Free Research Field

口腔衛生学

Academic Significance and Societal Importance of the Research Achievements

MRONJ発症に関わるNf-κBを制御する酸化ストレス発現の分子基盤として核内における酸化ストレス発症因子であるChgAとNrf2の相互関係が細胞実験により明らかになった。Nrf2タンパクを利用した酸化ストレス可視化マウスを用いた骨粗鬆症薬による顎骨壊死の今後の研究に繋がると考える。