Development and research of function maintenance culture method and new autologous transplantation method of human salivary gland cells

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K19186
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Chiba University
• Principal Investigator: FUKUSHIMA REO 千葉大学, 医学部附属病院, 医員 (00833576)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 口唇腺 / 頭頸部放射線治療 / 唾液腺機能回復 / 高度滅菌培養 / first in human / 唾液腺長期安定培養法

Outline of Final Research Achievements

After completion of our cell preparation protocol, optimal procedures of cell transportation and cell culture were examined in the Cell Processing Center (CPC) of Chiba University Hospital. We determined the appropriate cell number from labial glands for the transplantation, and then performed the transplantation with atelocollagen into human submandibular gland via catheter device for not only three healthy volunteers but also one patient after radiation therapy (RT) for oral cancer. Since we obtained problems of catheter insertion procedure for the patient with RT, such as trismus and small amounts of saliva, we will improve them for the First in Human study of transplantation of labial gland cells.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

今回、健常ボランティア3名と放射線治療終了後の患者1名にカテーテル挿入を行うことができた。それにより、カテーテル挿入の手技の確立やプロトコールの修正を行うことができ、カテーテル挿入時の問題点などを抽出することができた。また、CPC内で使用する細胞調製手順書/記録書が整い、CPCでの細胞培養を行う準備が整った。この結果をもとに今後、自己唾液腺細胞を用いた、唾液腺機能再生療法の研究をさらに進めていきたい。