Establishment of minimally invasive cleft palate closure treatment using mesenchymal stem cell-derived exosome from human exfoliated deciduous teeth

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K19269
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Hiroshima University
• Principal Investigator: Abe Takaharu 広島大学, 病院(歯), 助教 (20806682)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: MSC / SHED / 骨再生 / SHED-CM / エクソソーム

Outline of Final Research Achievements

In order to reduce the invasion of cleft lip and/or palate palate patients' secondary grafting, a study of bone regeneration therapy using deciduous pulp-derived mesenchymal stem cells (SHED) was conducted. In the first year, it was clarified that good bone regeneration can be achieved by transplanting carrier (atelocollagen) and SHED conditioned media (SHED-CM)into a bone defect prepared in a mouse skull.
In the next year, in order to improve the transplantation efficiency, CD146-positive, which is known as a cell adhesion molecule, was isolated from SHED and applied to transplantation. As a result, it was clarified that significantly good bone regeneration was produced. Furthermore, analysis of exosomes in SHED-CM is progressing, focusing on microRNAs contained in the factors.

Free Research Field

骨再生

Academic Significance and Societal Importance of the Research Achievements

近年乳歯歯髄由来間葉系幹細胞は着目されているものの、その詳細な性質については不明な点が多い。本研究では、SHED中に含有される液性成分について解析し、また、その成分を移植に応用することで、良好な骨再生を生じることを明らかとした。
本研究で用いたSHEDを応用し更なる骨再生機構の解明を試みることで、歯科医学にとどまらず広く再生医療分野の進歩へ貢献を果たすと考えられる。さらに、本法を臨床応用し患者の負担が軽減し、低侵襲で良好な骨再生療法を確立することは、社会的観点から重要な意義がある。