2023 Fiscal Year Final Research Report
Can dipeptidyl peptidase be an biomarker in the treatment of apical periodontitis?
| Project/Area Number |
19K19273
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57070:Developmental dentistry-related
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2024-03-31
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| Keywords | 歯周病原性菌 / ジペプチジルペプチダーゼ / DPP |
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| Outline of Final Research Achievements |
In the present study, we used a two-step cleavage method with glutamate-specific protease (GluV8) and DPP11 from Staphylococcus aureus strain V8 to examine the effect of prime-side residues on the peptidation of tetra-peptidyl-4-methylcoumaryl-7-amide (MCA), a substrate with amino acid residues bound at the P2-P2' position. We found a potential elevation of exopeptidase activity of Glu-specific endopeptidase I/GluV8 mediated by hydrophobic P1'-position amino acid residue. We also investigated the infection pathway of furcal lesions of deciduous teeth by micro-CT analysis.
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| Free Research Field |
小児歯科学
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| Academic Significance and Societal Importance of the Research Achievements |
我々の研究における口腔サンプルを用いたDPP活性測定により，歯周病原性菌の存在の有無が予測されたことから，DPP活性測定は歯周病原性菌のバイオマーカーとして有用であることが示唆されている．我々の研究対象であるdpp遺伝子の一部は，ヒトにも存在することが既知であり，ペプチド分解によって惹起される疾患の制御に繋がる可能性を持つ．本研究成果は，歯周疾患だけでなくこれら全身疾患への疾患予防・治療法確立への将来的な寄与が期待される．
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