Detection of serum microRNA that reflect chronic periodontitis

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K19314
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57080:Social dentistry-related
• Research Institution: Okayama University
• Principal Investigator: Yoneda Toshiki 岡山大学, 歯学部, 客員研究員 (60756071)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 慢性歯周炎 / microRNA

Outline of Final Research Achievements

The purpose of this study is to explore serum miRNAs which reflect periodontal condition. We enrolled 30 healthy patients without periodontitis and 30 patients with chronic periodontitis. Serum miRNA profiles were compared in samples from participants with and without chronic periodontitis using Microarray and Real-time PCR. Hsa-miR-664a-3p, hsa-miR-501-5p, and hsa-miR-21-3p are candidate serum biomarkers for chronic periodontitis. Bioinformatics study showed that the target genes of miR-664a-3p and miR-21-3p were strongly associated with MAPK signaling pathways, which play a role in the progression of diabetes mellitus.

Free Research Field

予防歯科学

Academic Significance and Societal Importance of the Research Achievements

本研究の学術的な特色は、microRNAを切り口にして、歯周病を反映したバイオマーカーを探索することにある。特に、①血液に注目して歯周病のバイオマーカーを探索する点、②歯周病重症度とmicroRNAの発現に関連があることを明らかにする点、などが挙げられる。
本研究の成果は、歯周状態を反映するバイオマーカーを開発するための重要な知見となり、歯周病の早期発見の診断技術の向上にも貢献できるかもしれない。